Our Division of Scientific Support is currently looking for a Post-doctoral fellow for developing an experimental pipeline for characterising membrane protein function in contrast-matched nano-carriers using Small Angle Neutron Scattering with in-situ size size-exclusion chromatography (SEC-SANS).

This role is part of the LINXS EU COFUND research project AMBER, Advanced Multiscale Biological imaging using European Research infrastructures.

Description of the workplace

You will be based in the Scientific Support Division of the European Spallation Source led by Hanna Wacklin-Knecht, where you will mainly work with developing methods for the deuterium labeling, purification and characterisation of contrast-matched lipid nanodiscs, and reconstitution of functional membrane proteins for neutron scattering experiments. You will collaborate closely with the Loki SANS instrument team to set-up the experimental workflows for in-situ size exclusion chromatography to characterize well-defined nanodisc samples.

The project is carried out in collaboration with Anne Martel at the Institut Laue Langevin in Grenoble, France, and includes training on the D22 instrument on the nanodisc, membrane protein reconstitution and SEC-SANS methodologies established there. For the protein expression, nanodisc purification and characterisation work, you will work at the Lund Protein Production Platform LP3 at Lund University where the ESS biological deuteration activities are co-hosted. You will also have access to a wide range of characterization techniques for nanodics samples at the Division of Physical Chemistry at Lund University, where Hanna Wacklin-Knecht holds an affiliation.

In addition to carrying out some of the earliest experiments on the Loki SANS instrument at ESS, proposal-based access to European neutron and X-ray beam facilities will also be used in this project.